Development of a Novel Recombinant Heamagglutinin-Neuramindase Elisa (rHN-ELISA) for Evaluation of Humoral Immunity in Chicken Vaccinated Against Newcastle Disease Virus (NDV)

A recombinant Newcastle disease virus (NDV) expressing VP2 protein of infectious bursal disease virus (IBDV) protects against NDV and IBDV.

Infectious bursal disease virus (IBDV) causes a highly immunosuppressive disease in chickens. Currently available, live IBDV vaccines can lead to generation of variant viruses. We have developed an alternative vaccine that will not create variant IBDV. By using the reverse genetics approach, we devised a recombinant Newcastle disease virus (NDV) vector from a commonly used vaccine strain LaSota...

ELISA for detection of humoral immunity against measles

Measles is a highly infectious and contagious disease of the respiratory system caused by Morbilivirus (Hashiguchi et al., 2011). The clinical disease has been characterized by high fever , cough, conjunctivitis, coryza, malaise, maculopapular rash and erythematous patches throughout the body along with increased mortality among non-vaccinated children (Ellison, 1931; Fazlalipour et al., 2008)....

Characterisation of genotype VII Newcastle disease virus (NDV) isolated from NDV vaccinated chickens, and the efficacy of LaSota and recombinant genotype VII vaccines against challenge with velogenic NDV

A Newcastle disease virus (NDV) isolate designated IBS002 was isolated from a commercial broiler farm in Malaysia. The virus was characterised as a virulent strain based on the multiple basic amino acid motif of the fusion (F) cleavage site (112)RRRKGF(117) and length of the C-terminus extension of the hemagglutinin-neuraminidase (HN) gene. Furthermore, IBS002 was classified as a velogenic NDV ...

Construction of Recombinant Bacmid DNA Encoding Newcastle Disease Virus (NDV) Fusion Protein Gene

Background and Aims: Newcastle disease virus (NDV) is one of the major pathogen in poultry. Vaccination is intended to control the disease as an effective solution nevertheless this virus is a growing threat to the poultry industry. F gene open reading frame (ORF) from NDV is 1650 bp, encoding a protein of 553 amino acids that can induce protective immunity alone. The F glycoprotein on the surf...

Antigen for indirect ELISA for infection with Newcastle disease virus